lineage tracing of mammary epithelial cells using cell-type-specific cre-expressing adenoviruses

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ID: 196239
2014
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Ranked #75 of 101 articles by views in nature reviews gastroenterology & hepatology

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Abstract
Lineage tracing using Cre/lox transgenic mice provides a powerful tool for studying normal mammary epithelial cell (MEC) development and the cellular origins of mammary tumors under physiological settings. However, generation of new transgenic mice for lineage-tracing purposes is often time consuming. Here, we report a lineage-tracing tool for MECs based on intraductal injection of lineage-specific Cre-expressing adenovirus (Ad-Cre). Using well-characterized promoters for Keratin 8 and Keratin 14, we generated lineage-specific Ad-Cre lines for luminal and basal MECs, respectively. By pulse-chase lineage tracing using these Ad-Cre lines, we showed that luminal and basal lineages are largely self-sustained and that IRS1 and IRS2 are essential for maintaining the basal lineage; we also showed that heterogeneous mammary tumors can be induced from luminal MECs in mice carrying the Etv6-NTRK3 fusion gene. Overall, we validated the Ad-Cre system as a promising and efficient tool for fate mapping of normal and malignant cells in adult tissues.
Reference Key
tao2014stemlineage Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Luwei Tao;Maaike P.A. van Bragt;Elizabeth Laudadio;Zhe Li
Journal nature reviews gastroenterology & hepatology
Year 2014
DOI
10.1016/j.stemcr.2014.04.004
URL
http://www.sciencedirect.com/science/article/pii/S2213671114001118
Keywords
medicine (general)biology (general)

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