identificação de cultivares e certificação da pureza genética de gladíolo por meio de marcadores morfológicos identification of cultivars and certification of genetic purity of gladiolus through morphologic markers

Clicks: 207
ID: 195981
2011
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Abstract
A caracterização de cultivares de flores é fundamental nos processos de registro e proteção dessas e também na certificação da pureza genética. Nesse trabalho foram realizadas a caracterização e a certificação da pureza genética de cultivares de Gladiolus sp. por meio de marcadores morfológicos. Utilizaram-se onze cultivares de Gladiolus sp., e para a caracterização das cultivares, as parcelas foram constituídas de 25 plantas avaliando-se características dos cormos, folhas e flores. O delineamento experimental adotado foi de blocos casualizados, com quatro repetições Para a certificação da pureza genética, foram instaladas parcelas constituídas de 24 plantas e cormos contendo contaminação varietal em diferentes proporções. Foram utilizadas três cultivares para compor os tratamentos e as avaliações foram conduzidas por três avaliadores que desconheciam a porcentagem existente de contaminação varietal. Foram calculadas as porcentagens de acerto e de erro com base nos resultados obtidos nas avaliações. Foi calculado o desvio padrão da média dos resultados nas quatro repetições, obtidos pelos três avaliadores. Para verificar a significância dos desvios entre os resultados obtidos pelos avaliadores foi utilizado o teste de qui-quadrado ( χ²). Por meio de características do cormo é possível diferenciar quatro cultivares das onze avaliadas. A característica da cor das flores é o descritor morfológico mais seguro para a diferenciação das cultivares de gladíolo. Dependendo das características das cultivares contaminantes e das cultivares em análises é possível utilizar descritores morfológicos para a certificação de pureza genética em cultivares de gladíolo.
The characterization of flower cultivars is fundamental in the registration and protection processes and also in the genetic purity certification. In this work, both the characterization and the genetic purity certification of cultivars of Gladiolus sp by means of morphologic markers were made. Eleven cultivars of Gladiolus sp. were used and for the characterization of the cultivars, the plots were made up of 25 plants, evaluating the characteristics of corms, leaves and flowers. The used experimental design was the one of randomized blocks with four replicates. For the genetic purity certification, plots with 24 plants and corms containing varietal contaminations in different proportions were established. Three cultivars were used to make up the treatments, and the evaluations were conducted by three evaluators who were unaware of the existent percentage of varietal contamination. The percentage of mistakes was calculated based upon the obtained results of the evaluations. The standard deviation of the means of the results in the four replicates obtained by the three evaluators was calculated. In order to verify the significance of the deviations among the obtained results by the evaluators, Chi-square test was used. By means of corm characteristics, it is possible to distinguish four cultivars out of the eleven evaluated. The characteristic of flower color is the safest morphological descriptor for distinguishing gladiolus cultivars. Depending on the characteristics of the contaminant cultivars and on the ones under analyses, it is possible to use morphological descriptors for the genetic purity certification in gladiolus cultivars.
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Authors ;Clarissa Alves Ferreira;Édila Vilela de Resende Von Pinho;Patrícia Duarte de Oliveira Paiva;Gabriella Santos Pereira;Rafael Parreira Diniz
Journal information and software technology
Year 2011
DOI
10.1590/S1413-70542011000400007
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000400007
Keywords
flowers

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