changes of enzyme activities in lens after glaucoma trabecular resection

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ID: 195441
2013
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Abstract
AIM: To observe the change of lens antioxidant enzyme activity after glaucoma trabecular resection.

METHODS: Thirty-two eyes of sixteen New-Zealand rabbits(2.2-2.4kg)were divided into two groups. The left eyes of rabbits underwent standard glaucoma trabecular resection were treatment group, and the normal right eyes served as controls. Transparency of lenses was monitored by a slit-lamp biomicroscopy before and after glaucoma trabecular resection. The morphology of lens cells was observed under the light microscope.The activities of Na+-K+-ATPase,catalase(CAT), glutathion peroxidase(GSH-px), glutathione reductase(GR), superoxide dismutase(SOD)and content of malondialdehyde(MDA)in lenses were detected six months after trabecular resection.

RESULTS: Lenses were clear in both treatment group and normal control group during the six months after operation. The morphology and structure of lens cells were normal under the light microscope in both operation group and normal group. The activity of lens cells antioxidant enzyme activity were significantly decreased in operation group compared with control group, Na+-K+-ATPase declined by 20.97%, CAT declined by 16.36%, SOD declined by 4.46%, GR declined by 4.85%, GSH-px declined by 10.02%, and MDA increased by 16.31%.

CONCLUSION: Glaucoma trabecular resection can induce the change of Na+-K+-ATPase, CAT, GSH-px, GR, SOD and MDA in lens of rabbit. Glaucoma filtration surgery for the occurrence of cataract development mechanism has important guiding significance.

Reference Key
wang2013guojichanges Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Jian-Ping Wang;Yong Ma;Yu-Shun Xue;Xuan-Yi Che;De-Xiu Zhang;Tao Zhu;Jing Li;Rui Shi;Gui-E Zhao
Journal journal of fungi
Year 2013
DOI
10.3980/j.issn.1672-5123.2013.08.07
URL
http://www.ies.net.cn/cn_publish/2013/8/201308007.pdf
Keywords
superoxide dismutase catalase lens

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