discrepant gene expression profile and phenotype changes in human lens epithelial cells after oxidative damage

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ID: 195057
2016
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Abstract
AIM:To explore the discrepant gene expression profiles and the related phenotype changes in human lens epithelial cells(HLEC)after oxidative stimulation.

METHODS:Human lens epithelial cell line(HLE-B3)were cultured in normal condition or with H2O2 for 24h. Total RNA were extracted for gene expression profiling assay and gene ontology analysis was performed for the significantly up-regulated genes using bioinformational database DAVID. The elevated expressions of up-regulated genes in HLEC after oxidative stimulation were confirmed by RT-qPCR. The apoptosis of HLEC induced by oxidative damage was detected using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT)assay and flow cytometry.

RESULTS:Gene expression profiling assay demonstrated that 367 genes were up-regulated in HLEC after oxidative stimulation. These genes were enriched in 310 biological processes mainly associated with p53-related signaling pathways, apoptosis, programed cell death and etc. Six genes mainly pro- or anti-apoptotic, including BCL2A1,TP53I3,FAS,ZMAT3,DDB2 and BCL2L1, were confirmed to be up-regulated by oxidative stimulation using RT-qPCR(P<0.05). Results of MTT assay and flow cytometry showed that apoptosis of HLEC gradually appeared after cells were treated with H2O2 and became the main consequence of oxidative stimulation.

CONCLUSION:Oxidative stimulation can induce up-regulation of proapoptotic genes and lead to apoptosis of HLEC, even though antiapoptotic genes can also be promoted.

Reference Key
mei2016guojidiscrepant Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Lin Mei;Song Wang;Bo Yan;An-Gang Yang;Jing Zhao;Hong Yan
Journal journal of fungi
Year 2016
DOI
10.3980/j.issn.1672-5123.2016.5.07
URL
http://ies.ijo.cn/cn_publish/2016/5/201605007.pdf
Keywords
oxidative damage apoptosisophthalmology

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