cordycepin induces s phase arrest and apoptosis in human gallbladder cancer cells

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ID: 192588
2014
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Abstract
Gallbladder cancer is the most common malignant tumor of the biliary tract, and this condition has a rather dismal prognosis, with an extremely low five-year survival rate. To improve the outcome of unresectable and recurrent gallbladder cancer, it is necessary to develop new effective treatments and drugs. The purpose of the present study was to evaluate the effects of cordycepin on human gallbladder cells and uncover the molecular mechanisms responsible for these effects. The Cell Counting Kit-8 (CCK-8) and colony formation assays revealed that cordycepin affected the viability and proliferation of human gallbladder cancer cells in a dose- and time-dependent manner. Flow cytometric analysis showed that cordycepin induced S phase arrest in human gallbladder cancer cell lines(NOZ and GBC-SD cells). Cordycepin-induced apoptosis was observed using an Annexin V/propidium iodide (PI) double-staining assay, and the mitochondrial membrane potential (ΔΨm) decreased in a dose-dependent manner. Additionally, western blot analysis revealed the upregulation of cleaved-caspase-3, cleaved-caspase-9, cleaved-PARP and Bax and the downregulation of Bcl-2, cyclin A and Cdk-2 in cordycepin-treated cells. Moreover, cordycepin inhibited tumor growth in nude mice bearing NOZ tumors. Our results indicate that this drug may represent an effective treatment for gallbladder carcinoma.
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wang2014moleculescordycepin Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Xu-An Wang, Shan-Shan Xiang, Huai-Feng Li, Xiang-Song Wu, Mao-Lan Li, Yi-Jun Shu, Fei Zhang, Yang Cao, Yuan-Yuan Ye, Run-Fa Bao, Hao Weng, Wen-Guang Wu, Jia-Sheng Mu, Yun-Ping Hu, Lin Jiang, Zhu-Jun Tan, Wei Lu, Ping Wang, Ying-Bin Liu
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Journal Journal of ethnopharmacology
Year 2014
DOI
10.3390/molecules190811350
URL
http://www.mdpi.com/1420-3049/19/8/11350
Keywords
proliferation cell cycle

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