generation of a pxr reporter human induced pluripotent stem cell line (pxr-mcherry hipsc) using the crispr/cas9 system

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ID: 192152
2018
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Abstract
Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.
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kim2018stemgeneration Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
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Journal journal of energy chemistry
Year 2018
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