scalable generation of universal platelets from human induced pluripotent stem cells
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ID: 192123
2014
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Abstract
Human induced pluripotent stem cells (iPSCs) provide a potentially replenishable source for the production of transfusable platelets. Here, we describe a method to generate megakaryocytes (MKs) and functional platelets from iPSCs in a scalable manner under serum/feeder-free conditions. The method also permits the cryopreservation of MK progenitors, enabling a rapid “surge” capacity when large numbers of platelets are needed. Ultrastructural/morphological analyses show no major differences between iPSC platelets and human blood platelets. iPSC platelets form aggregates, lamellipodia, and filopodia after activation and circulate in macrophage-depleted animals and incorporate into developing mouse thrombi in a manner identical to human platelets. By knocking out the β2-microglobulin gene, we have generated platelets that are negative for the major histocompatibility antigens. The scalable generation of HLA-ABC-negative platelets from a renewable cell source represents an important step toward generating universal platelets for transfusion as well as a potential strategy for the management of platelet refractoriness.
| Reference Key |
feng2014stemscalable
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| Authors | ;Qiang Feng;Namrata Shabrani;Jonathan N. Thon;Hongguang Huo;Austin Thiel;Kellie R. Machlus;Kyungho Kim;Julie Brooks;Feng Li;Chenmei Luo;Erin A. Kimbrel;Jiwu Wang;Kwang-Soo Kim;Joseph Italiano;Jaehyung Cho;Shi-Jiang Lu;Robert Lanza |
| Journal | nature reviews gastroenterology & hepatology |
| Year | 2014 |
| DOI |
10.1016/j.stemcr.2014.09.010
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