Entrepreneurship is one of the possible solutions to the pan-European problem of youth unemployment. The objective of the research was to identify differences between male and female entrepreneurs in their opinions of entrepreneurship of young people. We wanted to find out whether male and female entrepreneurs would recommend young people to do business and what factors in their opinion can influence the fact whether young people start business. To collect data, we used a questionnaire sent to entrepreneurs from the whole Czech Republic. The most important barrier identified by entrepreneurs was frequently amended legislation, complexity of administration, poor enforceability of law and a lack of funds. The most important incentives of young people according to entrepreneurs is particularly the vision of opportunities in business. Another important incentive is becoming more independent. Statistically significant differences in the responses of men and women were found in the barrier related to the personal knowledge of an unsuccessful entrepreneur and in the incentive of the necessity to start business due to the lack of a job. Sufficient funding and possessing business premises were factors in which statistically significant differences in the responses of men and women were found, too. This research emphasises factors which women and men can find important in making a decision on starting business or being employed.