molecular diagnosis of canine leproid granuloma out of paraffin-embedded histological cuts using polymerase chain reaction (pcr) – a retrospective study (2002-2009)
Article Quality & Performance Metrics
Readership in this journal
SteadyRanked #41 of 225 articles by views in frontiers in neuroendocrinology
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 225 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
Abstract
Canine leproid granuloma or “canine leprosy” is a mycobacteriosis that has a dermatopathic framework, which includes aspects that are still unclear, especially regarding its mode of transmission and etiological agent. In order to verify the genetic characterization of the mycobacteria that is involved in the condition, the present study analyzed histological cuts from animals with an established diagnosis of such disorder. The study samples were taken from of 13 dogs that were assisted at the HOVET / USP Dermatology Service. This material was subsequently subjected to “nested” and real-time polymerase chain reaction (PCR). The nested PCR confirmed the presence of the agent in 18.7% of the animals, whereas the real-time PCR detected 69.2% of samples with DNA of the mycobacterium, thus proving that such method presents higher sensitivity. Furthermore, it was observed that the species of mycobacterium that causes Canine Leproid Granulnoma in dogs from Brazil presents a 100% genetic homology when compared to samples obtained from animals in studies abroad.
| Reference Key |
maruyama2015semina:molecular
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Simoni Maruyama;Paulo Eduardo Brandão;Alessandra Marnie Martins Gomes de Castro;Nilceo Schwery Michalany;Janet Fyfe;Richard Malik;Carlos Eduardo Larsson |
| Journal | frontiers in neuroendocrinology |
| Year | 2015 |
| DOI |
10.5433/1679-0359.2015v36n5p3129
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.