quantitative analysis of human pluripotency and neural specification by in-depth (phospho)proteomic profiling

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ID: 188468
2016
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Ranked #72 of 101 articles by views in nature reviews gastroenterology & hepatology

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Abstract
Controlled differentiation of human embryonic stem cells (hESCs) can be utilized for precise analysis of cell type identities during early development. We established a highly efficient neural induction strategy and an improved analytical platform, and determined proteomic and phosphoproteomic profiles of hESCs and their specified multipotent neural stem cell derivatives (hNSCs). This quantitative dataset (nearly 13,000 proteins and 60,000 phosphorylation sites) provides unique molecular insights into pluripotency and neural lineage entry. Systems-level comparative analysis of proteins (e.g., transcription factors, epigenetic regulators, kinase families), phosphorylation sites, and numerous biological pathways allowed the identification of distinct signatures in pluripotent and multipotent cells. Furthermore, as predicted by the dataset, we functionally validated an autocrine/paracrine mechanism by demonstrating that the secreted protein midkine is a regulator of neural specification. This resource is freely available to the scientific community, including a searchable website, PluriProt.
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singec2016stemquantitative Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Ilyas Singec;Andrew M. Crain;Junjie Hou;Brian T.D. Tobe;Maria Talantova;Alicia A. Winquist;Kutbuddin S. Doctor;Jennifer Choy;Xiayu Huang;Esther La Monaca;David M. Horn;Dieter A. Wolf;Stuart A. Lipton;Gustavo J. Gutierrez;Laurence M. Brill;Evan Y. Snyder
Journal nature reviews gastroenterology & hepatology
Year 2016
DOI
10.1016/j.stemcr.2016.07.019
URL
http://www.sciencedirect.com/science/article/pii/S2213671116301436
Keywords
medicine (general)biology (general)

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