efficient precision genome editing in ipscs via genetic co-targeting with selection

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ID: 187819
2017
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Abstract
Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-mediated editing event at one locus enriches for HDR-mediated edits at additional loci. This strategy, called co-targeting with selection, improves the probability of isolating cells bearing HDR-mediated variants and accelerates the production of disease models.
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mitzelfelt2017stemefficient Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Katie A. Mitzelfelt, Chris McDermott-Roe, Michael N. Grzybowski, Maribel Marquez, Chieh-Ti Kuo, Michael Riedel, Shuping Lai, Melinda J. Choi, Kurt D. Kolander, Daniel Helbling, David P. Dimmock, Michele A. Battle, Chuanchau J. Jou, Martin Tristani-Firouzi, James W. Verbsky, Ivor J. Benjamin, Aron M. Geurts
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Journal nature reviews gastroenterology & hepatology
Year 2017
DOI
10.1016/j.stemcr.2017.01.021
URL
http://www.sciencedirect.com/science/article/pii/S2213671117300371
Keywords
medicine (general)biology (general)

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