efficient precision genome editing in ipscs via genetic co-targeting with selection
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ID: 187819
2017
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Abstract
Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-mediated editing event at one locus enriches for HDR-mediated edits at additional loci. This strategy, called co-targeting with selection, improves the probability of isolating cells bearing HDR-mediated variants and accelerates the production of disease models.
| Reference Key |
mitzelfelt2017stemefficient
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|---|---|
| Authors | ;Katie A. Mitzelfelt;Chris McDermott-Roe;Michael N. Grzybowski;Maribel Marquez;Chieh-Ti Kuo;Michael Riedel;Shuping Lai;Melinda J. Choi;Kurt D. Kolander;Daniel Helbling;David P. Dimmock;Michele A. Battle;Chuanchau J. Jou;Martin Tristani-Firouzi;James W. Verbsky;Ivor J. Benjamin;Aron M. Geurts |
| Journal | nature reviews gastroenterology & hepatology |
| Year | 2017 |
| DOI |
10.1016/j.stemcr.2017.01.021
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