efeitos de meios de cultura na formação de calos a partir de anteras de aspargo (asparagus officinalis l.) effects of the culture media on the calli formation from asparagus officinalis l. anthers

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ID: 187358
1995
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Abstract
Técnicas auxiliares, como a cultura de anteras, possibilitam melhor eficiência na obtenção de novos genótipos de aspargo. Neste trabalho, foram utilizadas anteras contendo grãos de pólen, no estágio uninucleado, dos híbridos 56x22-8 e 47x22-8, em meio MS, semi-sólido e líquido, com diferentes concentrações de reguladores de crescimento: meio A - 0,5 mg/l BAP + 0,1 mg/l ANA + 0,5 mg/l 2,4-D + 20 g/l sacarose + 20 g/l glicose; meio B - 0,5 mg/l 2,4-D + 30 g/l sacarose; meio C - 3,0 mg/l ANA + 0,5 mg/l Kin + 30 g/l sacarose e meio D - 2,0 mg/l ANA + 0,5 mg/l Kin + 30 g/l sacarose. A formação de calos variou com o genótipo e o meio. Os meios líquidos apresentaram melhor eficiência na indução de calos do que os meios semi-sólidos. Para o híbrido 56x22-8 os melhores meios foram o A2 (líquido) com 80,5% de indução de calos e dentre os meios semi-sólidos, o meio D1 com 41,6% de calos formados. Para o híbrido 47x22-8, o mais eficiente foi o meio D2 (líquido), com 68,1% de calos formados e, dentre os meios semi-sólidos, o D2, com 15,2%. Não ocorreram diferenças significativas, estatisticamente, entre os meios líquidos nem entre os meios semi-sólidos.
Anther culture is an auxiliary technique to obtain new genotypes. In this work, anthers with pollen grains in the uninucleated stage of hybrids 56x22-8 and 47x22-8 were cultured in MS medium, semi-solid and liquid, with different concentrations of growth regulators: medium A - 0,5 mg/l BAP + 0,1 mg/l NAA + 0,5 mg/1 2,4-D + 20 g/l sucrose + 20 g/l glucose; medium B - 0,5 mg/1 2,4-D + 30 g/l sucrose; medium C - 3,0 mg/l NAA + 0,5 mg/l Kin + 30 g/l sucrose and medium D - 2,0 mg/l NAA + 0,5 mg/l Kin + 30 g/l sucrose. Callus formation varied with the genotype and the medium. Liquid media showed more efficiency in callus development than the semi-solids. For the hybrids 56x22-8 the best media were the liquid A2 with 80,5% of callus induction and among the semi-solids the D1, with 41,6%. For the hybrid 47x22-8 were liquid D2 with 68,1% and semi solid D1 with 15,2%. No significant differences were observed among the liquid media nor among the semi-solidones.
Reference Key
bobrowski1995actaefeitos Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Vera Lúcia Bobrowski;José Antônio Peters;Eliane Augustin;Judith Viégas
Journal monatshefte für chemie chemical monthly
Year 1995
DOI
10.1590/S0102-33061995000100005
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33061995000100005
Keywords
anther culture

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