development and validation of analytical method for naftopidil in human plasma by lc–ms/ms

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ID: 187346
2015
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Abstract
A highly sensitive and simple high-performance liquid chromatographic–tandem mass spectrometric (LC–MS-MS) assay is developed and validated for the quantification of Naftopidil in human plasma. Naftopidil is extracted from human plasma by methyl tertiary butyl ether and analyzed using a reversed-phase gradient elution on a discovery C 18 5 μ (50 × 4.6) column. A methanol: 2 mM ammonium formate (90:10) as mobile phase, is used and detection was performed by MS using electrospray ionization in positive mode. Propranolol is used as the internal standard. The lower limits of quantification are 0.495 ng/mL. The calibration curves are linear over the concentration range of 0.495–200.577 ng/mL of plasma for each analyte. This novel LC–MS-MS method shows satisfactory accuracy and precision and is sufficiently sensitive for the performance of pharmacokinetic studies in humans.
Reference Key
jain2015arabiandevelopment Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Pritam S. Jain;Kajal D. Bobade;Pankaj R. Bari;Devendra S. Girase;Sanjay J. Surana
Journal Behavioural brain research
Year 2015
DOI
10.1016/j.arabjc.2013.06.020
URL
http://www.sciencedirect.com/science/article/pii/S1878535213001846
Keywords
lc–ms/ms human plasma

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