identification of anti-persister activity against uropathogenic escherichia coli from a clinical drug library

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ID: 187282
2015
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Abstract
Uropathogenic E. coli is a major cause of urinary tract infections (UTIs), but current antibiotics do not always effectively clear the persistent infection. To identify drugs that eliminate uropathogenic E. coli persisters, we screened a clinical drug library consisting of 1524 compounds using high throughput drug exposure assay in 96-well plates. Bacterial survival was assessed by growth on LB plates. We identified 14 drug candidates (tosufloxacin, colistin, sparfloxacin, moxifloxacin and gatifloxacin, enrofloxacin and sarafloxacin, octodrine, clofoctol, dibekacin, cephalosporin C, pazufloxacin, streptomycin and neomycin), which had high anti-persister activity. Among them, tosufloxacin and colistin had the highest anti-persister activity and could completely eradicate E. coli persisters in 3 days in vitro while the current UTI antibiotics failed to do so. Our findings may have implications for the development of a more effective treatment for UTIs.
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niu2015antibioticsidentification Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Hongxia Niu;Peng Cui;Wanliang Shi;Shuo Zhang;Jie Feng;Yong Wang;David Sullivan;Wenhong Zhang;Bingdong Zhu;Ying Zhang
Journal derecho pucp
Year 2015
DOI
10.3390/antibiotics4020179
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