changes in the microrna profile observed in the subcutaneous adipose tissue of obese patients after laparoscopic adjustable gastric banding

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ID: 186765
2017
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Abstract
Background. Laparoscopic adjustable gastric banding (LAGB) results in significant lasting weight loss and improved metabolism in obese patients. To evaluate whether epigenetic factors could concur to these benefits, we investigated the subcutaneous adipose tissue (SAT) microRNA (miRNA) profile before (T0) and three years (T1) after LAGB in three morbidly obese women. Case Reports. SAT miRNA profiling, evaluated by TaqMan Array, showed four downexpressed (miR-519d, miR-299-5p, miR-212, and miR-671-3p) and two upexpressed (miR-370 and miR-487a) miRNAs at T1 versus T0. Bioinformatics predicted that these miRNAs regulate genes belonging to pathways associated with the cytoskeleton, inflammation, and metabolism. Western blot analysis showed that PPAR-alpha, which is the target gene of miR-519d, increased after LAGB, thereby suggesting an improvement in SAT lipid metabolism. Accordingly, the number and diameter of adipocytes were significantly higher and lower, respectively, at T1 versus T0. Bioinformatics predicted that the decreased levels of miR-212, miR-299-5p, and miR-671-3p at T1 concur in reducing SAT inflammation. Conclusion. We show that the miRNA profile changes after LAGB. This finding, although obtained in only three cases, suggests that this epigenetic mechanism, by regulating the expression of genes involved in inflammation and lipid metabolism, could concur to improve SAT functionality in postoperative obese patients.
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nardelli2017journalchanges Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Carmela Nardelli;Laura Iaffaldano;Vincenzo Pilone;Giuseppe Labruna;Maddalena Ferrigno;Nicola Carlomagno;Concetta Anna Dodaro;Pietro Forestieri;Pasqualina Buono;Francesco Salvatore;Lucia Sacchetti
Journal chinese chemical letters
Year 2017
DOI
10.1155/2017/6754734
URL
http://dx.doi.org/10.1155/2017/6754734
Keywords
internal medicine

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