induction of avirulence by avr-pita1 in virulent u.s. field isolates of magnaporthe oryzae
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ID: 186104
2014
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Abstract
The AVR-Pita1 gene, from the Chinese isolate O-137 of Magnaporthe oryzae, is an effector that determines the efficacy of the Pi-ta rice blast resistance gene. In the present study, the avirulence function of AVR-Pita1 was induced by transformation of field isolates (TM2, ZN19, B2 and B8) that originally were collected from the U.S. and are virulent on Pi-ta-carrying rice cultivars. The presence of AVR-Pita1 from O-137 in independent transformants was detected by PCR using AVR-Pita1 specific primers and verified by DNA sequencing and Southern blot analysis using the AVR-Pita1 coding region as a probe. The results of pathogenicity assays showed that the AVR-Pita1-transformed isolates were not able to infect rice cultivars Katy and Drew carrying Pi-ta. Control isolates that were transformed with inserts lacking the AVR-Pita1 gene remained virulent. Our findings demonstrate that AVR-Pita1 can be used to induce novel gene-specific blast resistance in nature.
| Reference Key |
dai2014cropinduction
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| Authors | ;Yuntao Dai;Eugenia Winston;James C. Correll;Yulin Jia |
| Journal | Biomaterials science |
| Year | 2014 |
| DOI |
doi:10.1016/j.cj.2013.12.002
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