functional and pharmacological analysis of cardiomyocytes differentiated from human peripheral blood mononuclear-derived pluripotent stem cells

Clicks: 192
ID: 185552
2014
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Abstract
Advances in induced pluripotent stem cell (iPSC) technology have set the stage for routine derivation of patient- and disease-specific human iPSC-cardiomyocyte (CM) models for preclinical drug screening and personalized medicine approaches. Peripheral blood mononuclear cells (PBMCs) are an advantageous source of somatic cells because they are easily obtained and readily amenable to transduction. Here, we report that the electrophysiological properties and pharmacological responses of PBMC-derived iPSC CM are generally similar to those of iPSC CM derived from other somatic cells, using patch-clamp, calcium transient, and multielectrode array (MEA) analyses. Distinct iPSC lines derived from a single patient display similar electrophysiological features and pharmacological responses. Finally, we demonstrate that human iPSC CMs undergo acute changes in calcium-handling properties and gene expression in response to rapid electrical stimulation, laying the foundation for an in-vitro-tachypacing model system for the study of human tachyarrhythmias.
Reference Key
riedel2014stemfunctional Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Michael Riedel;Chuanchau J. Jou;Shuping Lai;Robert L. Lux;Alonso P. Moreno;Kenneth W. Spitzer;Elizabeth Christians;Martin Tristani-Firouzi;Ivor J. Benjamin
Journal nature reviews gastroenterology & hepatology
Year 2014
DOI
10.1016/j.stemcr.2014.04.017
URL
http://www.sciencedirect.com/science/article/pii/S2213671114001246
Keywords
medicine (general)biology (general)

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