hepatoprotectivitve effect of iranian grape seed and jaft (a part of oak fruit) extracts against ccl4 induced-liver toxicity in rats

Background: Specialists are more interest to grape seed oil for antioxidant activity. Flavonoid compounds, particularly proanthocyanidin are mainly responsible for antioxidant potential in grape seed oil. In addition, recently the interest in using natural remedies to treat diseases increases the use of herbal drugs.The protective effect of grape seed and Jaft extract (a part of oak fruit) was studied on liver toxicity which induced by carbon tetra chloride (CCl4) in rats. Methods: This study carried out on 28 male wistar rats. Mixture of hydroalchoholic grape seed and Jaft extracts was gavaged (200mg/kg) in two groups of extract control and experiment groups, daily for 7 days. At the same time, in two groups of CCl4(toxic ) and treatment, CCl4 in olive oil solution was injected (1ml/kg i.p) since third day, daily, for 5 days. In control groups, olive oil injected (0.5ml/kg i.p) daily, for 5 days. Results: There was significant (P≤0.05) increase in the hepatic enzyme levels such as Aspartate Transaaminase (AST), Alanine Transaminase (ALT), Alkaline Phosphates (ALP) and Bilirubin in toxic group compare to control group. Treat with extract can reduce elevation of liver enzymes AST, ALT, ALP and bilirubin, that caused by CCl4 toxin. The administered of extract only, did not meaningfully alter the enzyme levels when compared to the control groups. Conclusion: According to damage induced by CCl4 and protective potential of extract, we can recommend that mixture of grape seed and Jaft extract has hepatoprotective effect due to antioxidant components.