experimental ophitoxemia produced by the opisthoglyphous lora snake (philodryas olfersii) venom ofitoxemia experimental produzida pelo veneno da serpente opistoglifa lora (philodryas olfersii)

Clicks: 766
ID: 184110
2006
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Abstract
Several colubrid snakes produce venomous oral secretions. In this work, the venom collected from Venezuelan opisthoglyphous (rear-fanged) Philodryas olfersii snake was studied. Different proteins were present in its venom and they were characterized by 20% SDS-PAGE protein electrophoresis. The secretion exhibited proteolytic (gelatinase) activity, which was partially purified on a chromatography ionic exchange mono Q2 column. Additionally, the haemorrhagic activity of Philodryas olfersii venom on chicken embryos, mouse skin and peritoneum was demonstrated. Neurotoxic symptoms were demonstrated in mice inoculated with Philodryas olfersii venom. In conclusion, Philodryas olfersii venom showed proteolytic, haemorrhagic, and neurotoxic activities, thus increasing the interest in the high toxic action of Philodryas venom.
Várias serpentes da família Colubridae produzem secreções orais venenosas. Neste trabalho, foi estudado o veneno coletado da presa posterior da serpente opistóglifa venezuelana Philodryas olfersii. Deferentes proteínas estavam presentes no veneno, sendo caracterizadas pela eletroforese de proteínas (SDS-PAGE) a 20%. A secreção mostrou atividade proteolítica (gelatinase) a qual foi parcialmente purificada em uma coluna de intercâmbio iônico (mono Q2). Adicionalmente, a atividade hemorrágica do veneno de Philodryas olfersii foi demonstrada em embriões de galinha, pele e peritônio de rato. Os sintomas neurológicos foram demonstrados em camundongos inoculados com veneno de Philodryas olfersii. Em conclusão, o veneno da Philodryas olfersii mostrou atividade proteolítica, hemorrágica, e neurotóxica, assim aumentando o interesse na elevada ação tóxica do veneno da Philodryas olfersii.
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rodrguez-acosta2006revistaexperimental Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Alexis Rodríguez-Acosta;Karel Lemoine;Luis Navarrete;María E. Girón;Irma Aguilar
Journal mobile information systems
Year 2006
DOI
10.1590/S0037-86822006000200012
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822006000200012
Keywords
haemorrhage tropical medicine venomarctic medicine

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