simultaneous determination of pioglitazone and candesartan in human plasma by lc-ms/ms and its application to a human pharmacokinetic study

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ID: 183615
2012
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Abstract
A simple and rapid liquid chromatography-tandem mass spectrometric (LC-MS/MS) assay method has been developed and fully validated for simultaneous quantification of pioglitazone and candesartan in human plasma. Irbesartan was used as an internal standard. The analytes were extracted from human plasma samples by solid-phase extraction technique using a Strata-X 33 μm polymeric sorbent. The reconstituted samples were chromatographed on a C18 column by using a 80:20 (v/v) mixture of acetonitrile and 0.1% formic acid as the mobile phase at a flow rate of 0.8 mL/min. The calibration curves obtained were linear (r≥0.99) over the concentration range of 15–3000 ng/mL for pioglitazone and 5–608 ng/mL for candesartan. The results of the intra- and inter-day precision and accuracy studies were well within the acceptable limits. A run time of 2.7 min for each sample made it possible to analyze more than 300 plasma samples per day. The proposed method was found to be applicable to clinical studies. Keywords: Pioglitazone, Candesartan, Human plasma, Solid-phase extraction, LC-MS/MS, Pharmacokinetics
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karra2012journalsimultaneous Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Vijaya Kumari Karra;Nageswara Rao Pilli;Jaswanth Kumar Inamadugu;J.V.L.N. Seshagiri Rao
Journal Frontiers in plant science
Year 2012
DOI
DOI not found
URL
http://www.sciencedirect.com/science/article/pii/S2095177912000123
Keywords
therapeutics pharmacology

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