síndrome respiratorio agudo grave: el laboratorio de salud pública frente a una emergencia global severe acute respiratory syndrome: the public health laboratory in a global emergency

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ID: 183160
2005
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Abstract
A fines del año 2002 se inicia un brote de neumonía atípica en el Sudeste asiático el cual se extiende posteriormente a otros continentes. El nuevo síndrome respiratorio agudo grave (SARS) era producido por un coronavirus novedoso. Debido a la gravedad de la situación y al riesgo de introducción de esta patología en Argentina, se implementaron técnicas de diagnóstico clásicas como la microscopía electrónica, y moleculares como una reacción de retrotranscripción seguida de una reacción en cadena de la polimerasa (RT-PCR). La inclusión en araldita de células infectadas con un coronavirus bovino permitió visualizar más fácilmente las partículas virales, pero requirió más tiempo en comparación con la coloración negativa de partículas libres de cultivos virales. La RT-PCR implementada fue capaz de detectar ARN de cepas de casos de Hong Kong y de Alemania.
By the end of year 2002 there was an outbreak of atypical pneumonia in Southeast Asia which soon spread to other continents. This new severe acute respiratory syndrome (SARS) was produced by a novel coronavirus. Due to the severity of the situation and risk of introduction of this pathology in our country, the need to arrange specific laboratory diagnostic tests arose. Classic techniques, such as the electron microscopy and molecular biology test such as retrotranscription followed by the polymerase chain reaction (RT-PCR) were implemented. The araldit included cells infected with bovine coronavirus which allowed the viral particles to be visualized easily but it took more time in comparison with the negative staining of free particles from viral cultures. RT-PCR was able to detect RNA of isolated viruses from cases in Hong Kong and Germany.
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baumeister2005medicinasndrome Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Elsa G. Baumeister, Adrian P. Lewis, Juan P. Bozzini, Vilma L. Savy
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Journal proceedings on: 2016 ieee central america and panama student conference, conescapan 2016
Year 2005
DOI
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URL
http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0025-76802005000100006
Keywords
pcr sars allergyinfectious and parasitic diseases

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