non-symbiotic bacterial diazotrophs from of agricultural crops of san carlos. córdoba, colombia
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ID: 182262
2007
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Abstract
Diazotrophic Azotobacter sp and Azospirrillum sp bacteria were isolated from the rhizosphere of plantain, (Musa paradisiaca), corn, wheat and fallow areas (uncultivated, but covered by fallen leaves) in the San Carlos region (in the Sinú valley in the Córdoba department, Colombia). Microbial populations were identified by biochemical tests; physiological characters were examined using Gram reaction in different mediums: Burk’s, Ashby and Jensen’s (Azotobacter sp) and Burk’s, NFB and Congo-red medium (Azospirillum sp). The study was aimed at determining ion ammonium production from Azotobacter sp and Azospirrillum sp isolates; ion ammonium was quantified according to Berthelot’s colorimetric technique (phenol-hypochlorite). This method was modified and standardised in line with using a Perkin-Elmer Lamba 11 UV-Vis spectrometer. As a result of this study, 14 isolates have been shown to be ammonium-producers at concentrations ranging from 0.9 mg/l to 5.2 mg/l; A16PG (5.1545 mg/l) and A26M1P (5.1743 mg/l) yielded the highest ammonium concentrations for Azotobacter sp and A5M1G (4.6741 mg/l) for Azospirrillum sp. Biological N2 fixation (BNF) by associative diazotrophic bacteria has contributed towards increasing harvest yield, thereby reducing the need for nitrogenised fertilisers and the emission of greenhouse gases (such as N2O) and obtaining economic and environmental benefits for farming.
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mantilla2007revistanon-symbiotic
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| Authors | ;Cecilia Lara Mantilla;Mara Villalba Anaya;Luis Eliécer Oviedo Zumaqué |
| Journal | gigascience |
| Year | 2007 |
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