temperature and uv light affect the activity of marine cell-free enzymes
Clicks: 75
ID: 181998
2017
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Emerging Content
22.2
/100
75 views
2 readers
AI Quality Assessment
Not analyzed
Readership in this journal
EmergingRanked #318 of 345 articles by views in tetrahedron letters
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 345 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Microbial extracellular enzymatic activity (EEA) is the
rate-limiting step in the degradation of organic matter in the oceans. These
extracellular enzymes exist in two forms: cell-bound, which are attached to
the microbial cell wall, and cell-free, which are completely free of the cell.
Contrary to previous understanding, cell-free extracellular enzymes make up a
substantial proportion of the total marine EEA. Little is known about these
abundant cell-free enzymes, including what factors control their activity
once they are away from their sites (cells). Experiments were run to assess
how cell-free enzymes (excluding microbes) respond to ultraviolet radiation
(UVR) and temperature manipulations, previously suggested as potential
control factors for these enzymes. The experiments were done with New Zealand
coastal waters and the enzymes studied were alkaline phosphatase (APase),
β-glucosidase, (BGase), and leucine aminopeptidase (LAPase).
Environmentally relevant UVR (i.e. in situ UVR levels measured at our site)
reduced cell-free enzyme activities by up to 87 % when compared
to controls, likely a consequence of photodegradation. This effect of UVR on
cell-free enzymes differed depending on the UVR fraction. Ambient levels of
UV radiation were
shown to reduce the activity of cell-free enzymes for
the first time. Elevated temperatures (15 °C) increased the activity
of cell-free enzymes by up to 53 % when compared to controls
(10 °C), likely by enhancing the catalytic activity of the enzymes.
Our results suggest the importance of both UVR and temperature as control
mechanisms for cell-free enzymes. Given the projected warming ocean
environment and the variable UVR light regime, it is possible that there could be
major changes in the cell-free EEA and in the enzymes contribution to
organic matter remineralization in the future.
| Reference Key |
thomson2017biogeosciencestemperature
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;B. Thomson;C. D. Hepburn;M. Lamare;F. Baltar;F. Baltar |
| Journal | tetrahedron letters |
| Year | 2017 |
| DOI |
10.5194/bg-14-3971-2017
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.