the haemoculture of trypanosoma minasense chagas, 1908

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ID: 181926
1996
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Abstract
Trypanosoma minasense was isolated for the first time in blood axenic culture from a naturally infected marmoset, Callithrix penicillata, from Brazil. The parasite grew profusely in an overlay of Roswell Park Memorial Institute medium plus 20% foetal bovine serum, on Novy, McNeal and Nicolle medium (NNN) , at 27°C, with a peak around 168 hr. The morphometry of cultural forms of T. minasense, estimates of cell population size and comparative growth in four different media overlays always with NNN, were studied. The infectivity of cultural forms to marmosets (C. penicillata and C. jacchus) and transformation of epimastigotes into metacyclic-like forms in axenic culture in the presence of chitin derivates (chitosan) were evaluated.
Reference Key
ziccardi1996memriasthe Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Mariangela Ziccardi;Ricardo Lourenço-de-Oliveira;Ricardo Nogueira
Journal kurdistan journal of applied research
Year 1996
DOI
10.1590/S0074-02761996000400019
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