analysis of the growth pattern of vero cells cultured on dense and porous poly (l-lactic acid) scaffolds

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ID: 181541
2009
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Abstract
Poly (L-lactic acid) (PLLA) polymers are the most frequently used substrates for cell culture, tissue regeneration and orthopedic prostheses, mainly because of their atoxic characteristics and good biocompatibility. The objective of this study was to evaluate whether a higher density or different pore diameters (less than 45, 180-250, and 250-350 µm) would change the growth pattern of cultured cells. The cells were found to adhere to and spread over all PLLA scaffolds studied. The cells also showed similar proliferation on dense and porous PLLA scaffolds, except for PLLA scaffolds with a smaller pore diameter. The cytochemical data showed high metabolic cellular activity on the various substrates. Overall, the results indicated satisfactory cell growth and proliferation on the different PLLA scaffolds studied, especially for those with pore diameters of 180-250 µm and 250-350 µm.
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Authors ;Arnaldo Rodrigues Santos Jr;Samuel Hilsdorf Barbanti;Eliana Aparecida de Rezende Duek;Maria Lucia Furlan Wada
Journal Majalah Ilmiah Teknologi Elektro
Year 2009
DOI
10.1590/S1516-14392009000300002
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392009000300002
Keywords
scaffolds cell culture mechanics of materials tissue engineeringmaterials of engineering and construction

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