quantitative analysis of γ–oryzanol content in cold pressed rice bran oil by tlc–image analysis method

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ID: 180788
2014
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Abstract
Objective: To develop and validate an image analysis method for quantitative analysis of γ-oryzanol in cold pressed rice bran oil. Methods: TLC-densitometric and TLC-image analysis methods were developed, validated, and used for quantitative analysis of γ-oryzanol in cold pressed rice bran oil. The results obtained by these two different quantification methods were compared by paired t-test. Results: Both assays provided good linearity, accuracy, reproducibility and selectivity for determination of γ-oryzanol. Conclusions: The TLC-densitometric and TLC-image analysis methods provided a similar reproducibility, accuracy and selectivity for the quantitative determination of γ-oryzanol in cold pressed rice bran oil. A statistical comparison of the quantitative determinations of γ-oryzanol in samples did not show any statistically significant difference between TLC-densitometric and TLC-image analysis methods. As both methods were found to be equal, they therefore can be used for the determination of γ-oryzanol in cold pressed rice bran oil.
Reference Key
sakunpak2014asianquantitative Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Apirak Sakunpak, Jirapornchai Suksaeree, Chaowalit Monton, Pathamaporn Pathompak, Krisana Kraisintu
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Journal Organic letters
Year 2014
DOI
10.1016/S2221-1691(14)60219-7
URL
http://www.sciencedirect.com/science/article/pii/S2221169115301805
Keywords
tropical medicinebiology (general)

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