quantitative analysis of γ–oryzanol content in cold pressed rice bran oil by tlc–image analysis method
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ID: 180788
2014
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Abstract
Objective: To develop and validate an image analysis method for quantitative analysis of γ-oryzanol in cold pressed rice bran oil.
Methods: TLC-densitometric and TLC-image analysis methods were developed, validated, and used for quantitative analysis of γ-oryzanol in cold pressed rice bran oil. The results obtained by these two different quantification methods were compared by paired t-test.
Results: Both assays provided good linearity, accuracy, reproducibility and selectivity for determination of γ-oryzanol.
Conclusions: The TLC-densitometric and TLC-image analysis methods provided a similar reproducibility, accuracy and selectivity for the quantitative determination of γ-oryzanol in cold pressed rice bran oil. A statistical comparison of the quantitative determinations of γ-oryzanol in samples did not show any statistically significant difference between TLC-densitometric and TLC-image analysis methods. As both methods were found to be equal, they therefore can be used for the determination of γ-oryzanol in cold pressed rice bran oil.
| Reference Key |
sakunpak2014asianquantitative
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|---|---|
| Authors | ;Apirak Sakunpak;Jirapornchai Suksaeree;Chaowalit Monton;Pathamaporn Pathompak;Krisana Kraisintu |
| Journal | Organic letters |
| Year | 2014 |
| DOI |
10.1016/S2221-1691(14)60219-7
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