examining the impact of acetylene on n-fixation and the active sediment microbial community

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ID: 180634
2015
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Ranked #524 of 875 articles by views in journal of magnetic resonance (san diego, calif : 1997)

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Abstract
Here we examined the impact of a commonly employed method used to measure nitrogen fixation, the acetylene reduction assay (ARA), on a marine sediment community. Historically, the ARA technique has been broadly employed for its ease of use, in spite of numerous known artifacts. To gauge the severity of these effects in a natural environment, we employed high-throughput sequencing to detect differences in acetylene-treated sediments versus non-treated control sediments after a seven hour incubation. Within this short time period, significant differences were seen broadly across all types of microbes identified in the sediment, implying that the changes induced by acetylene occur quickly. The results have important implications for our understanding of marine nitrogen budgets. Moreover, because the ARA technique has been widely used in terrestrial and freshwater habitats, these results may be applicable to other ecosystems.
Reference Key
fulweiler2015frontiersexamining Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Robinson W (Wally) Fulweiler;Elise M. Heiss;Elise M. Heiss;Mary Kate eRogener;Mary Kate eRogener;Silvia E. Newell;Silvia E. Newell;Gary R. LeCleir;Sarah eKortebein;Steven W. Wilhelm
Journal journal of magnetic resonance (san diego, calif : 1997)
Year 2015
DOI
10.3389/fmicb.2015.00418
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