effet de la pectolyase y-23 et de la cellulase onozuka rs sur le rendement en protoplastes viables de prunus cerasus l.
Clicks: 337
ID: 180520
2001
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Popular Article
30.2
/100
337 views
41 readers
AI Quality Assessment
Not analyzed
Readership in this journal
PopularRanked #6 of 42 articles by views in proceedings of 2018 ieee international conference on mechatronics and automation, icma 2018
Most read
Least read
Bar heights use a square-root scale.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Effect of pectolyase Y-23 and cellulase Onozuka RS on the yield of viable protoplasts of Prunus cerasus L. ""Montmorency"". To isolate leaf mesophyll, leaf and root callus protoplasts of Prunus cerasus L. ""Montmorency"", we have determined the optimum enzymatic mixtures to be used, and characterized the specific activity of these enzymes. The analysis of the specific activities of enzymes allows to compare the different cellulases and pectinases used to obtain protoplasts in relation with the tissue sources. This analysis concerned the FPase (degradation of filter paper) and CMCase activities for cellulases Onozuka RS and R-10, and the PME (pectinmethylesterase), PL (pectate lyase) and PG (polygalacturonase) activities for the pectinases Macerozyme R-10 and Pectolyase Y-23. The results show that the digestion of leaf mesophyll tissues need cellulase Onozuka RS and Pectolyase Y-23 while callus protoplasts of the same material, can be isolated with cellulase Onozuka R-10 and Macerozyme R-10. The enzymes cellulase Onozuka RS and Pectolyase Y-23 (as pectinase) improved significantly the yield and the viability of leaf mesophyll protoplasts compared to cellulase Onozuka R-10 and Macerozyme R-10. These results were correlated to the specific activities of the enzymes. Significant differences between the 2 pectinases are observed for PME, PL and PG activities and between the 2 cellulases for CMCase activity. From callus, the maximum amount of viable protoplasts was obtained with cellulase Onozuka R-10 (low CMCase activity) and Macerozyme R-10 (low PG activity).
| Reference Key |
r.2001biotechnologie,effet
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Mehri-Kamoun R. |
| Journal | proceedings of 2018 ieee international conference on mechatronics and automation, icma 2018 |
| Year | 2001 |
| DOI |
DOI not found
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.