derivation of dm1 affected human embryonic stem cell line genea067
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2016
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Abstract
The Genea067 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying expansion of CTG repeats in the DMPK gene, indicative of Myotonic Dystrophy Type 1 (DM1). Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XY and STR analysis demonstrated a male Allele pattern. The hESC line had pluripotent cell morphology, 85% of cells expressed Nanog, 97% Oct4, 73% Tra1-60 and 98% SSEA4 and gave a Pluritest Pluripotency score of 25.75, Novelty of 1.46. The cell line was negative for Mycoplasma and visible contamination.
| Reference Key |
dumevska2016stemderivation
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| Authors | ;Biljana Dumevska;Heather Main;Robert McKernan;Divya Goel;Uli Schmidt |
| Journal | journal of energy chemistry |
| Year | 2016 |
| DOI |
10.1016/j.scr.2016.02.014
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