a mutagenesis assay for reporter gene screening using partially degenerate oligonucleotides of the tandems nnt and nnc

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ID: 179079
2015
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Ranked #227 of 1,324 articles by views in spectrochimica acta - part a: molecular and biomolecular spectroscopy

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Abstract
Not all proteins are tolerable to mutations. Whether a specific protein can be a mutable target is of importance in the biotechnology and pharmaceutical industry. This study reported a novel mutagenesis assay using tandem NNT and NNC oligonucleotides to test the mutability of a candidate gene. These two tandem oligonucleotides avoid the risk of forming nonsense mutations and render flexibility of truncating or expanding the insertion size. As a reporter gene, ZeoR (zeocin resistance gene) was confirmed to have a high tolerance for mutagenesis by this new assay.
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xu2015biomeda Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
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Journal spectrochimica acta - part a: molecular and biomolecular spectroscopy
Year 2015
DOI
10.1155/2015/950873
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