a mutagenesis assay for reporter gene screening using partially degenerate oligonucleotides of the tandems nnt and nnc
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ID: 179079
2015
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Abstract
Not all proteins are tolerable to mutations. Whether a specific protein can be a mutable target is of importance in the biotechnology and pharmaceutical industry. This study reported a novel mutagenesis assay using tandem NNT and NNC oligonucleotides to test the mutability of a candidate gene. These two tandem oligonucleotides avoid the risk of forming nonsense mutations and render flexibility of truncating or expanding the insertion size. As a reporter gene, ZeoR (zeocin resistance gene) was confirmed to have a high tolerance for mutagenesis by this new assay.
| Reference Key |
xu2015biomeda
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|---|---|
| Authors | ;Huifen Xu;Cuilan Zhou;Andy K. Zhang;Wen Li;Jia Zhang;Kai Li |
| Journal | spectrochimica acta - part a: molecular and biomolecular spectroscopy |
| Year | 2015 |
| DOI |
10.1155/2015/950873
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