rapid detection of salmonella in food and beverage samples by polymerase chain reaction

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ID: 178915
2010
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Abstract
Polymerase chain reaction (PCR) assay had been used to detect Salmonella in food and beverage samples using suitable primers which are based on specific invA gene of Salmonella. Twenty nine samples were collected from street food counters and some canteens in Margonda Street, Depok, West Java, Indonesia. It was found that five of twenty nine samples were detected to contain Salmonella and showed the presence of the amplified product of the size 244 bp. The method of PCR demonstrated the specificity of invA primers for detection of Salmonella as confirmed by biochemical and serological assay. The results of this study revealed that PCR was a rapid and useful tool for detection of Salmonella in food and beverage samples.
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m.2010malaysianrapid Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Radji, M.;Malik, A.;Widyasmara, A.
Journal Journal of developmental origins of health and disease
Year 2010
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