measuring bacterial cells size with afm

Clicks: 283
ID: 178897
2012
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Abstract
Atomic Force Microscopy (AFM) can be used to obtain high-resolution topographical images of bacteria revealing surface details and cell integrity. During scanning however, the interactions between the AFM probe and the membrane results in distortion of the images. Such distortions or artifacts are the result of geometrical effects related to bacterial cell height, specimen curvature and the AFM probe geometry. The most common artifact in imaging is surface broadening, what can lead to errors in bacterial sizing. Several methods of correction have been proposed to compensate for these artifacts and in this study we describe a simple geometric model for the interaction between the tip (a pyramidal shaped AFM probe) and the bacterium (Escherichia coli JM-109 strain) to minimize the enlarging effect. Approaches to bacteria immobilization and examples of AFM images analysis are also described.
Reference Key
osiro2012brazilianmeasuring Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Denise Osiro;Rubens Bernardes Filho;Odilio Benedito Garrido Assis;Lúcio André de Castro Jorge;Luiz Alberto Colnago
Journal ieee software
Year 2012
DOI
10.1590/S1517-83822012000100040
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100040
Keywords
Escherichia coli atomic force microscopy (afm)

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