generation of non-transgenic ips cells from human cord blood cd34+ cells under animal component-free conditions
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ID: 178759
2017
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Abstract
Recently, many hurdles and limitations for production of clinically applicable iPSC derivatives have been overcome. Transgene-free iPSCs can be efficiently derived from easily accessible cell sources such as blood. Here we describe the generation of transgene-free hiPS cells from cord blood derived CD34+ cells, reprogrammed using CytoTune™ Sendai reprogramming vectors. CD34+ cell isolation, cultivation, reprogramming and establishment of resulting hiPSC lines were performed under the exclusive usage of animal-derived component-free (ADCF) materials and components.
| Reference Key |
haase2017stemgeneration
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|---|---|
| Authors | ;Alexandra Haase;Gudrun Göhring;Ulrich Martin |
| Journal | journal of energy chemistry |
| Year | 2017 |
| DOI |
10.1016/j.scr.2017.03.022
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