generation of non-transgenic ips cells from human cord blood cd34+ cells under animal component-free conditions

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ID: 178759
2017
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Abstract
Recently, many hurdles and limitations for production of clinically applicable iPSC derivatives have been overcome. Transgene-free iPSCs can be efficiently derived from easily accessible cell sources such as blood. Here we describe the generation of transgene-free hiPS cells from cord blood derived CD34+ cells, reprogrammed using CytoTune™ Sendai reprogramming vectors. CD34+ cell isolation, cultivation, reprogramming and establishment of resulting hiPSC lines were performed under the exclusive usage of animal-derived component-free (ADCF) materials and components.
Reference Key
haase2017stemgeneration Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Alexandra Haase, Gudrun Göhring, Ulrich Martin
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Journal journal of energy chemistry
Year 2017
DOI
10.1016/j.scr.2017.03.022
URL
http://www.sciencedirect.com/science/article/pii/S1873506117300661
Keywords
Biology (General)

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