composição da matriz de encapsulamento na formação e conversão de sementes sintéticas de pimenta-longa composition of the encapsulation matrix on the formation and conversion of synthetic seeds of long pepper

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ID: 178353
2008
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Abstract
A pimenta-longa (Piper hispidinervium C. DC.) é uma planta nativa caracterizada pela produção de safrol, um metabólito secundário usado na produção de perfumes, cosméticos e inseticidas. Neste trabalho avaliou-se o uso potencial da tecnologia de sementes sintéticas como alternativa à propagação in vitro de pimenta-longa. Foi estudada a influência da constituição da cápsula sobre a formação e conversão in vitrode unidades encapsuláveis. Sementes pré-germinadas de pimenta-longa com 21 dias de cultivo em meio de cultura de MS foram utilizadas como material de encapsulamento. A concentração de alginato de sódio utilizada na matriz de encapsulamento foi de 1,5% (p/v) e a esta consistência se testou na água e o meio de MS nas concentrações plena (100%), 3/4 (75%) e 1/2 (50%) dos sais e vitaminas, associado ou não a carvão ativado (3 g L-1) como elementos da cápsula. Após encapsulamento, os cultivos foram mantidos em sala de crescimento a 25±2ºC, fotoperíodo de 16 horas e intensidade luminosa de 30 mmol m-2 s-1, onde a taxa de conversão e a altura das plantas foram avaliadas quinzenalmente até 30 dias de cultivo. O delineamento utilizado foi inteiramente casualizado, em fatorial 2 x 4, com cinco repetições e dez unidades encapsuláveis por parcela. Verificou-se que o emprego de um endosperma sintético constituído por 3/4 dos sais e vitaminas de MS, acrescido de carvão ativado (3 g L-1) (85,8% de taxa de conversão) ou a utilização de MS pleno, independente do uso de carvão ativado (76,5%) promoveram as melhores taxas de conversão de sementes sintéticas de pimenta-longa. A altura das plantas não foi afetada pelas concentrações de MS e adição de carvão ativado à cápsula.
Long pepper (Piper hispidinervumC. DC.) is a native plant characterized by the production of safrol, a secondary metabolite used in the production of perfumes, cosmetics and insecticides. In the present work the potential use of synthetic seeds technology as an alternative to in vitropropagation of long pepper was evaluated. The influence of capsule constitution on formation and conversion of in vitroencapsulated units was also studied. Long pepper pre-germinated 21-day old seeds were used as material for encapsulation. The concentration of sodium alginate used in the encapsulation matrix was 1,5% (p/v) and this consistency was tested in water or MS at full (100%), 3/4 (75%) and ½ (50%) concentration of salt and vitamins, associated or not to active charcoal (3 g L-1), as nutritional elements of the capsule. After encapsulation, cultures were maintained in a growth room at 25±2ºC and a photoperiod of 16 h at 30 mmol m-2s-1, where the conversion rate and height of the plants were evaluated for a month. The experimental design was of randomized blocks arranged in a 2 x 4 factorial scheme, with five replicates and ten encapsulated units for replicate. It was verified that The employment of a synthetic endosperm constituted by MS at ¾ concentration, enriched with active charcoal (3 g L-1) (85.8% of conversion) or MS at full concentration, independently of active charcoal (76.5%), promoted the best results on the conversion of synthetic seeds of long pepper. The height of the plants was not affected by the MS concentrations and addition of active charcoal to the capsule.
Reference Key
pereira2008horticulturacomposio Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Jonny ES Pereira;Rodrigo da S Guedes;Frederico Henrique da S Costa;Gottfried Cristian B Schmitz
Journal research on biomedical engineering
Year 2008
DOI
10.1590/S0102-05362008000100018
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362008000100018
Keywords
micropropagation sodium alginate

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