effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method
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ID: 177746
2012
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Abstract
This paper describes principles of a proper dilution of samples used for the determination of antioxidant capacity by means of a photochemiluminiscence method using the instrument Photochem (Analytik Jena AG). The authors used the method ACL (Lipid-soluble Antioxidant Capacity), which is one of two methods enabling to measure in this instrument. It was demonstrated that values measured by the photochemiluminiscence method ACL were influenced by the degree of sample dilution. When studying effects of dilution of samples of wine and rutin, it was demonstrated that there is a non-linear correlation between the degree of dilution and measured values of antioxidant capacity. At low molar amount of substance (i.e. at 0.5 and 1.0 nmol), the measured value of rutin inhibition was higher than the same that molar amount of trolox. At higher molar amounts (i.e. 3; 4 and 5 nmol), the inhibition value of trolox was higher. This dependent change can be explained by means of a different effectiveness of antioxidants and a different stability of their products with radicals.
| Reference Key |
hc2012actaeffect
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|---|---|
| Authors | ;Pavel Híc;Josef Balík |
| Journal | Talanta |
| Year | 2012 |
| DOI |
10.11118/actaun201260080067
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| URL | |
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