limit of detection of toxocara canis larvae in experimentally contaminated bovine milk
Article Quality & Performance Metrics
Readership in this journal
SteadyRanked #42 of 225 articles by views in frontiers in neuroendocrinology
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 225 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
Abstract
This study aimed to evaluate the limit of detection of Toxocara canis larvae in experimentally contaminated commercial bovine milk samples, based on a centrifuge-sedimentation technique. Firstly, bovine milk (whole and skim) samples were contaminated with 50 T. canis larvae in order to evaluate the interference of milk fat with the recovery of the larvae. Next, the effects of 10% formalin (100 ?L), ether (100 ?L), and a combination of both solutions on the recovery of the larvae was examined. Thereafter, the limit of detection of the larvae was determined using the solution (from step 2) considered optimal for degreasing the milk sample. Samples were contaminated with aliquots of 1, 5, 10, 25, and 50 larvae. For each milk sample (1.0 mL), 15 repetitions were analysed. The recovery of the larvae from the skim milk samples was higher (p = 0.0031) than that from the whole milk samples. No significant difference (p = 0.5681) was observed with regard to the percentage of recovered larvae when comparing the degreasing solutions. Nevertheless, the formalin-ether combination was more efficient for recovering the larvae (73.1%) than ether (71.9%), formalin (67.6%), and pure whole milk (70.0%). Concerning the limit of detection (using formalin-ether), all the samples contaminated with 5, 10, 25, and 50 larvae tested positive (minimum: 62.7%). Of the samples contaminated with a single larva, 66.7% tested positive. These results suggest that the centrifugation-sedimentation technique may be useful for recovering larvae of Toxocara spp. in naturally or experimentally contaminated milk samples obtained from a wide range of animal species.
| Reference Key |
pereira2016semina:limit
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;Edlayne Larissa Gretter Machado Pereira;Vamilton Alvares Santarém;Aline da Silveira Batista;Yslla Fitz Merigueti;Lundia Luara Cavalcante Bin |
| Journal | frontiers in neuroendocrinology |
| Year | 2016 |
| DOI |
10.5433/1679-0359.2016v37n1p213
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.