inhibitory effect of three c-glycosylflavonoids from cymbopogon citratus (lemongrass) on human low density lipoprotein oxidation

This study assessed the inhibitory effect of three C-glycosylflavonoids from Cymbopogon citratus leaves - isoorientin (1), swertiajaponin (2) and isoorientin 2"-Orhamnoside (3) - on human LDL oxidation. Isolated LDL was incubated with compounds 1-3 and the kinetics of lipid peroxidation were assessed by conjugated diene and malondialdehyde-thiobarbituric acid reactive substances (MDA-TBARS) formation after addition of copper ions. Significant differences (p < 0.05) between the lag time phase of the control and the lag time phase in the presence of the compounds 1 (0.25 µM) and 2 (0.50 µM) were observed. After five hours of incubation all three compounds showed a significant inhibitory effect on MDA-TBARS formation with respect to the control. After six hours of incubation only compound 1 kept a remarkable antioxidant effect. This study demonstrates that isoorientin (1) is an effective inhibitor of in vitro LDL oxidation. As oxidative damage to LDL is a key event in the formation of atherosclerotic lesions, the use of this natural antioxidant may be beneficial to prevent or attenuate atherosclerosis.