molecular and morphological identification of biomphalaria species from the state of são paulo, brazil

Clicks: 120
ID: 177049
2017
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Abstract
DNA barcoding and morphological characters were used to identify adult snails belonging to the genus Biomphalaria from 17 municipalities in the state of São Paulo, Brazil. The DNA barcode analysis also included twenty-nine sequences retrieved from GenBank. The final data set of 104 sequences of the mitochondrial cytochrome oxidase I (COI) gene was analyzed for K2P intraspecific and interspecific divergences, through tree-reconstruction methods (Neighbor-Joining, Maximum Likelihood and Bayesian inference), and by applying different models (ABGD, bPTP, GMYC) to partition the sequences according to the pattern of genetic variation. Twenty-seven morphological parameters of internal organs were used to identify specimens. The molecular taxonomy of Biomphalaria agreed with the morphological identification of specimens from the same collection locality. DNA barcoding may therefore be a useful supporting tool for identifying Biomphalaria snails in areas at risk for schistosomiasis.
Reference Key
palasio2017zookeysmolecular Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Raquel Gardini Sanches Palasio;Marisa Cristina de Almeida Guimarães;Fernanda Pires Ohlweiler;Roseli Tuan
Journal biochimica et biophysica acta
Year 2017
DOI
10.3897/zookeys.668.10562
URL
http://zookeys.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_xml&item_id=10562
Keywords
Zoology

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