simultaneous determination of five diterpenoid alkaloids in herba delphinii by hplc/elsd
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ID: 176756
2013
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Abstract
A HPLCâELSD method was developed and validated for simultaneous determination of five Hetisane-type diterpenoid alkaloids in a Tibetan traditional herbal medicine, âGebu Diluâ (Herba Delphinii), using a Kromasil C18 column (250 mmÃ4.6 mm, 5 μm) with the mobile phase consisting of acetonitrile and 0.1% triethylamine in gradient (detected by evaporative light scattering detector). The linear ranges of five compounds were determined and method validation was evaluated completely. The established method is rapid and accurate with high repeatability, and can be applied for the quality control of Herba Delphinii. Keywords: HPLCâELSD, Herba Delphinii, Hetisane-type diterpenoid alkaloids, Determination, Quality control
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| Reference Key |
lin2013journalsimultaneous
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| Authors | ;Chao-Zhan Lin;Si-Min Xie;Chen-Chen Zhu;Zeren-Dawa Bairu;Suolang-Qimei Kangsa;Dun Zhu |
| Journal | Frontiers in plant science |
| Year | 2013 |
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