probing temperature- and ph-dependent binding between quantum dots and bovine serum albumin by fluorescence correlation spectroscopy
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2017
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Abstract
Luminescent quantum dots (QDs) with unique optical properties have potential applications in bio-imaging. The interaction between QDs and bio-molecules is important to the biological effect of QDs in vivo. In this paper, we have employed fluorescence correlation spectroscopy (FCS) to probe the temperature- and pH-dependent interactions between CdSe QDs with carboxyl (QDs-COOH) and bovine serum albumin (BSA) in buffer solutions. The results have shown that microscopic dissociation constant K′D is in the range of (1.5 ± 0.2) × 10−5 to (8.6 ± 0.1) × 10−7 M, the Hill coefficient n is from 0.4 to 2.3, and the protein corona thickness is from 3.0 to 9.4 nm. Variable-temperature measurements have shown both negative values of ∆H and ∆S for BSA adsorption on QDs-COOH, while pH has a profound effect on the adsorption. Additional, FCS measurement QDs-COOH and proteins in whole mice serum and plasma samples has also been conducted. Finally, simulation results have shown four favored QD binding sites in BSA.
| Reference Key |
wang2017nanomaterialsprobing
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|---|---|
| Authors | ;Zonghua Wang;Qiyan Zhao;Menghua Cui;Shichao Pang;Jingfang Wang;Ying Liu;Liming Xie |
| Journal | progress in neuro-psychopharmacology & biological psychiatry |
| Year | 2017 |
| DOI |
10.3390/nano7050093
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