questioning conventional wisdom regarding the most suitable sequence of enzyme usage in pulp bleaching

Increased public scrutiny and governmental legislation towards the pulp and paper industries have motivated industrialists and researchers to seek improved bleaching sequences having the potential to minimize pollutants in bleach effluent generated during manufacturing of paper. Discovery of toxic chlorinated organics and their components in bleach effluents has focused people’s attention towards finding alternative ways of bleaching pulp. Use of enzymes at industrial scale has become well known, but still it is not clear whether the sequence of enzymatic treatment most often employed in industrial applications represents the best overall practice. The point of enzyme addition is critically important to maximize benefits. Many publications describe the use of an enzyme treatment stage before the use of chemicals in a bleaching process. Insufficient attention has been paid to the alternatives of adding an enzyme in between chemical bleaching agents (intermediate) or at the end of the bleaching process.