detection and characterization of hepatitis e virus in goats at slaughterhouse in tai’an region, china
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2017
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Abstract
Background. Hepatitis E virus (HEV) is a significant pathogen of viral hepatitis and can be transmitted through fecal-oral route. Epidemiological data concerning HEV in goats, however, are relatively sparse to date. Here, the prevalence and characteristics of HEV isolated from goats at slaughterhouse were investigated in Tai’an region, China. Methods. Anti-HEV immunoglobulin G (IgG) in blood samples and HEV RNA in the liver samples were determined by using an enzyme-linked immunosorbent assay (ELISA) and a nested reverse transcription polymerase chain reaction (RT-PCR), respectively. In addition, partial nucleotide sequences of open reading frame 2 (ORF-2) of HEV isolates were analyzed. Results. Fifty goat blood samples (46.7%, 50/120) were masculine for anti-HEV IgG. HEV RNA was detected in 2 liver samples (4.0%, 2/50) and belonged to genotype 4 subtype 4 h, with high identity (91.2–93%) with cow HEV strains detected in the same province, China. Conclusions. These findings demonstrated that goats may be an important reservoir for HEV and can become a major source of HEV infection in humans via food chain.
| Reference Key |
li2017biomeddetection
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| Authors | ;Song Li;Mingxia Liu;Jingjing Cong;Yufa Zhou;Zengmin Miao |
| Journal | spectrochimica acta - part a: molecular and biomolecular spectroscopy |
| Year | 2017 |
| DOI |
10.1155/2017/3723650
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