probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets
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ID: 174746
2018
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Abstract
We report an experimental approach to study the mechanosensitivity of cell-cell contact upon mechanical stimulation in suspended cell-doublets. The doublet is placed astride an hourglass aperture, and a hydrodynamic force is selectively exerted on only one of the cells. The geometry of the device concentrates the mechanical shear over the junction area. Together with mechanical shear, the system also allows confocal quantitative live imaging of the recruitment of junction proteins (e.g., E-cadherin, ZO-1, occludin, and actin). We observed the time sequence over which proteins were recruited to the stretched region of the contact. The compressed side of the contact showed no response. We demonstrated how this mechanism polarizes the stress-induced recruitment of junctional components within one single junction. Finally, we demonstrated that stabilizing the actin cortex dynamics abolishes the mechanosensitive response of the junction. Our experimental design provides an original approach to study the role of mechanical force at a cell-cell contact with unprecedented control over stress application and quantitative optical analysis.
| Reference Key |
gao2018aplprobing
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|---|---|
| Authors | ;Xumei Gao;Bipul R. Acharya;Wilfried Claude Otto Engl;Richard De Mets;Jean Paul Thiery;Alpha S. Yap;Virgile Viasnoff |
| Journal | international journal of civil engineering and technology |
| Year | 2018 |
| DOI |
10.1063/1.5025216
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| URL | |
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