structural characterization of lignin in wild-type versus comt down-regulated switchgrass
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ID: 174731
2014
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Abstract
This study examined the chemical structural characteristics of cellulolytic enzyme lignin isolated from switchgrass focusing on comparisons between wild-type control and caffeic acid 3-O-methyltransferase (COMT) down-regulated transgenic line. Nuclear magnetic resonance (NMR) techniques including 13C, 31P, and two-dimensional 13C-1H heteronuclear single quantum coherence (HSQC) as well as gel permeation chromatography (GPC) were employed. Compared to the wild-type, the COMT down-regulated transgenic switchgrass lignin demonstrated a decrease in syringyl (S): guaiacyl (G) ratio and p-coumarate:ferulate ratio, an increase in relative abundance of phenylcoumaran unit, and a comparable content of total free phenolic OH groups along with formation of benzodioxane unit. In addition, COMT down-regulation had no significant effects on the lignin molecular weights during its biosynthesis process.
| Reference Key |
eragauskas2014frontiersstructural
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|---|---|
| Authors | ;Arthur eRagauskas;Yunqiao ePu;Reichel eSamuel;Nan eJiang;Chunxiang eFu;Zeng-Yu eWang |
| Journal | geoheritage |
| Year | 2014 |
| DOI |
10.3389/fenrg.2013.00014
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| URL | |
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