high frequency of thermodesulfovibrio spp. and anaerolineaceae in association with methanoculleus spp. in a long-term incubation of n-alkanes-degrading methanogenic enrichment culture

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ID: 173708
2016
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Ranked #122 of 875 articles by views in journal of magnetic resonance (san diego, calif : 1997)

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Resumen
In the present study, the microbial community and functional gene composition of a long-term active alkane-degrading methanogenic culture was established after two successive enrichment culture transfers and incubated for a total period of 1750 days. Molecular analysis was conducted after the second transfer (incubated for 750 days) for both the active alkanes-degrading methanogenic enrichment cultures (T2-AE) and the background control (T2-BC). A net increase of methane as the end product was detected in the headspace of the enrichment cultures amended with long-chain n-alkanes and intermediate metabolites, including octadecanoate, hexadecanoate, isocaprylate, butyrate, isobutyrate, propionate, acetate and formate were measured in the liquid cultures. The composition of microbial community shifted through the successive transfers over time of incubation. Sequences of bacterial and archaeal 16S rRNA gene (16S rDNA) and mcrA functional gene indicated that bacterial sequences affiliated to Thermodesulfovibrio spp. and Anaerolineaceae and archaeal sequences falling within the genus Methanoculleus were the most frequently encountered and thus represented the dominant members performing the anaerobic degradation of long-chain n-alkanes and methanogenesis. In addition, the presence of assA functional genes encoding the alkylsuccinate synthase α subunit indicated that fumarate addition mechanism could be considered as a possible initial activation step of n-alkanes in the present study. The succession pattern of microbial communities indicates that Thermodesulfovibrio spp. could be a generalist participating in the metabolism of intermediates, while Anaerolineaceae plays a key role in the initial activation of long-chain n-alkane biodegradation.
Clave de Referencia
liang2016frontiershigh Use esta clave para autocitar en el manuscrito mientras usa SciMatic Manuscript Manager o Thesis Manager
Autores ;Bo Liang;Li-Ying Wang;Zhichao Zhou;Serge Maurice Mbadinga;Serge Maurice Mbadinga;Lei Zhou;Jin-Feng Liu;Jin-Feng Liu;Shi-Zhong Yang;Shi-Zhong Yang;Ji-Dong Gu;Bozhong Mu;Bozhong Mu
Revista journal of magnetic resonance (san diego, calif : 1997)
Ano 2016
DOI
10.3389/fmicb.2016.01431
URL
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01431/full
Palabras Clave
microbial community 16s rrna gene anaerobic methanogenesis

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