nuevos genotipos de arroz resistentes a la piriculariosis obtenidos por cultivo de anteras
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2012
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Abstract
Título en inglés: New blast resistant rice genotypes obtained by and anthers culture.
Resumen: En la Estación Experimental del Arroz Los Palacios, perteneciente al Instituto Nacional de Ciencias Agrícolas de Cuba (INCA), se efectuaron cruzamientos entre cuatro cultivares resistentes a la Piriculariosis y seis de buen comportamiento agronómico y las anteras de las plantas F2 fueron cultivadas in vitro para evaluar la formación de callos en tres medios líquidos: N6-1, N6-m y NL, así como la regeneración de plantas verdes y albinas, en el medio MS. Las dos primeras generaciones de las nuevas líneas obtenidas fueron evaluadas para caracteres agronómicos y la segunda generación, además, para resistencia frente a la Piriculariosis. Las líneas que combinaron resistencia a la Piriculariosis y buenos caracteres agronómicos fueron evaluadas en condiciones de infección natural, con alta presión del patógeno. La utilización de la técnica del cultivo de anteras mostró alta dependencia del genotipo y el medio de cultivo. Con el medio NL se lograron los valores más altos para la formación de callos. Se obtuvieron nuevos genotipos resistentes a la Piriculariosis y de alto rendimiento agrícola.
Palabras clave: Arroz; Mejoramiento genético; Cultivo in Vitro de anteras
Summary: Crosses were made between four blast resistant and six rice varieties of good agronomic performance, at the Los Palacios Rice Research Station of the National Agricultural Sciences Institute of Cuba (INCA) and the anthers from F2 plants were in vitro culture using three liquid media: N6-1, N6m, and NL, for callus formation and after plants regenerations using MS medium. The first two generations of breeding lines were evaluated for agronomic characters and the second generations, also, for Blast resistant. The lines that combined resistance to Blast and good agronomic performance were evaluated under high pressure of natural Blast infection conditions. The success rate of anther culture was highly dependent on the genotype and culture media used. NL medium led to the highest callus formation values. In the process, new blast resistant and high yielding genotypes were obtained.
Key words: Rice; Rice Breeding; Anther culture
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castro2012revistanuevos
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| Authors | ;Rodolfo I Castro;Noraida de Jesús Pérez;María Caridad González;Manuel Aguilar |
| Journal | gigascience |
| Year | 2012 |
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