development of gonadotropin-releasing hormone-secreting neurons from human pluripotent stem cells

Clics: 234
ID: 172840
2016
PDF Gratuit
Métriques de Qualité et de Performance de l'Article
Qualité Globale Improving Quality
0.0 /100
Combine les données d'engagement avec l'évaluation de la qualité académique par IA
Évaluation de la Qualité par IA
Non analysé
Résumé
Gonadotropin-releasing hormone (GnRH) neurons regulate human puberty and reproduction. Modeling their development and function in vitro would be of interest for both basic research and clinical translation. Here, we report a three-step protocol to differentiate human pluripotent stem cells (hPSCs) into GnRH-secreting neurons. Firstly, hPSCs were differentiated to FOXG1, EMX2, and PAX6 expressing anterior neural progenitor cells (NPCs) by dual SMAD inhibition. Secondly, NPCs were treated for 10 days with FGF8, which is a key ligand implicated in GnRH neuron ontogeny, and finally, the cells were matured with Notch inhibitor to bipolar TUJ1-positive neurons that robustly expressed GNRH1 and secreted GnRH decapeptide into the culture medium. The protocol was reproducible both in human embryonic stem cells and induced pluripotent stem cells, and thus provides a translational tool for investigating the mechanisms of human puberty and its disorders.
Clé de Référence
lund2016stemdevelopment Utilisez cette clé pour citer automatiquement dans le manuscrit lors de l'utilisation de SciMatic Manuscript Manager ou Thesis Manager
Auteurs ;Carina Lund;Kristiina Pulli;Venkatram Yellapragada;Paolo Giacobini;Karolina Lundin;Sanna Vuoristo;Timo Tuuri;Parinya Noisa;Taneli Raivio
Revue nature reviews gastroenterology & hepatology
Année 2016
DOI
10.1016/j.stemcr.2016.06.007
URL
http://www.sciencedirect.com/science/article/pii/S2213671116300996
Mots-clés
hypogonadotropic hypogonadism puberty induced pluripotent stem cells human embryonic stem cells gnrh gonadotropin-releasing hormone

Citations

Aucune citation trouvée. Pour ajouter une citation, contactez l'administrateur à info@scimatic.org

Commentaires

Aucun commentaire pour le moment. Soyez le premier à commenter cet article.