Epigallocatechin gallate and gallic acid affect colonization of abiotic surfaces by oral bacteria.

epigallocatechin gallate and gallic acid are known antimicrobial agents. Their roles in controlling microbial colonization, such as bacterial attachment and biofilm formation, are however not completely clear. This study aims to investigate their effects on the colonization of abiotic surfaces by oral bacteria and study the mechanism of their activities.the effects of epigallocatechin gallate and gallic acid on cell surface physicochemical properties (hydrophobicity and charge) of a range of oral bacteria and their auto-aggregation, attachment and biofilm formation on different abiotic surfaces (glass, stainless steel and hydroxyapatite) were studied.results show that epigallocatechin gallate inhibited bacterial attachment to the hard surfaces (except hydroxyapatite) by 0.2-1.4 log CFU cm by affecting cell surface hydrophobicity and charge. In addition, epigallocatechin gallate induced notches on cell surfaces of Streptococcus mutans without affecting their viability and biofilm formation. Gallic acid enhanced auto-aggregation (by 7.9-30.6 %) and biofilm formation by Actinomyces naeslundii (by 0.9-1.2 log CFU cm) by causing calcium efflux from the cells.the tested phytochemicals influenced the colonization of abiotic surfaces by oral bacteria through different mechanisms, most notably via affecting cell surface physicochemical properties, inducing changes in the shape of cell envelopes and causing calcium efflux.