expression of annexin-a1 and galectin-1 anti-inflammatory proteins and mrna in chronic gastritis and gastric cancer

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ID: 171047
2013
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Abstract
Objective. The anti-inflammatory proteins annexin-A1 and galectin-1 have been associated with tumor progression. This scenario prompted us to investigate the relationship between the gene and protein expression of annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) in an inflammatory gastric lesion as chronic gastritis (CG) and gastric adenocarcinoma (GA) and its association with H. pylori infection. Methods. We analyzed 40 samples of CG, 20 of GA, and 10 of normal mucosa (C) by the quantitative real-time PCR (qPCR) technique and the immunohistochemistry assay. Results. High ANXA1 mRNA expression levels were observed in 90% (36/40) of CG cases (mean relative quantification RQ = 4.26 ± 2.03) and in 80% (16/20) of GA cases (mean RQ = 4.38 ± 4.77). However, LGALS1 mRNA levels were high (mean RQ = 2.44 ± 3.26) in 60% (12/20) of the GA cases, while low expression was found in CG (mean RQ = 0.43±3.13; P<0.01). Normal mucosa showed modest immunoreactivity in stroma but not in epithelium, while stroma and epithelium displayed an intense immunostaining in CG and GA for both proteins. Conclusion. These results have provided evidence that galectin-1 and mainly annexin-A1 are overexpressed in both gastritis and gastric cancer, suggesting a strong association of these proteins with chronic gastric inflammation and carcinogenesis.
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jorge2013mediatorsexpression Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Yvana Cristina Jorge;Mayra Mioto Mataruco;Leandro Pires Araújo;Ana Flávia Teixeira Rossi;Juliana Garcia de Oliveira;Marina Curado Valsechi;Alaor Caetano;Kenji Miyazaki;Célia Sebastiana de Jesus Fazzio;Jorge Alberto Thomé;Paula Rahal;Sonia Maria Oliani;Ana Elizabete Silva
Journal polyhedron
Year 2013
DOI
10.1155/2013/152860
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