null alleles are ubiquitous at microsatellite loci in the wedge clam (donax trunculus)

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ID: 169925
2017
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Abstract
Recent studies have reported an unusually high frequency of nonamplifying alleles at microsatellite loci in bivalves. Null alleles have been associated with heterozygous deficits in many studies. While several studies have tested for its presence using different analytical tools, few have empirically tested for its consequences in estimating population structure and differentiation. We characterised 16 newly developed microsatellite loci and show that null alleles are ubiquitous in the wedge clam, Donax trunculus. We carried out several tests to demonstrate that the large heterozygous deficits observed in the newly characterised loci were most likely due to null alleles. We tested the robustness of microsatellite genotyping for population assignment by showing that well-recognised biogeographic regions of the south Atlantic and south Mediterranean coast of Spain harbour genetically different populations.
Reference Key
rico2017peerjnull Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Ciro Rico;Jose Antonio Cuesta;Pilar Drake;Enrique Macpherson;Louis Bernatchez;Amandine D. Marie
Journal pediatrics
Year 2017
DOI
10.7717/peerj.3188
URL
https://peerj.com/articles/3188/
Keywords
microsatellites null alleles

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